Ampflstr identifiler pcr amplification kit manual

The AmpFLSTR Identifiler PCR Amplification Kit is the single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems. The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards. AmpFlSTR Identifiler PCR Amplification Kit User’s Manual PCR Setup 1. Label PCR tubes with sample names. 2. For each µL reaction, add µL PCR reaction mix, µL AmpliTaq Gold DNA polymerase, and µL Identifiler primer set. AmpFlSTR®Identifiler® PCR Amplification Kit Mian Sahib Zar 1 *, Ahmad Ali Shahid, Muhammad Saqib Shahzad 2, Kyoung-Jin Shin 3, Hwan Young Lee 3 .

Shop All Products. Manuals User Guides. AmpFLSTR® Identifiler® Plus PCR Amplification Kit User Guide. Applied Biosystems™. AmpFLSTR® Identifiler® Plus PCR Amplification Kit User Guide. Catalog number: Related applications: Forensics. Technical Support Customer Service. Applied Biosystems™. Optimized for casework, the AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. Purpose The AmpFlSTR™ Identifiler™ PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that amplifies 15 tetranucleotide repeat loci and the Amelogenin gender-determining marker in a single PCR amplification: • All thirteen of the required loci for the Combined DNA Index System (CODIS) (Budowle et al., ).

AmpFℓSTR. ®. Identifiler. ®. Plus Amplification Kit (ID+) (Life cycles with a total volume of 25 µL per well (Applied Biosystems, 03/, User Guide). for 28 cycles as per the AmpFlSTR® Identifiler® PCR. Amplification Kit User's Manual. Amplification products were detected using the Applied Biosystems AmpFlSTR® Identifiler® Kit Storage: Upon receipt of AmpFlSTR® Identifiler® kits, use the PCR Amplification Kit Log to Forensic Biology Procedure Manual.